Identification of a functional cysteine essential for the activity of guinea pig liver transglutaminase.
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چکیده
منابع مشابه
Mechanism of Action of Guinea Pig Liver Transglutaminase I. PURIFICATION AND PROPERTIES OF THE ENZYME: IDENTIFICATION OF A FUNCTIONAL CYS- TEINE ESSENTIAL FOR ACTIVITY
Guinea pig liver transglutaminase has been purified 230fold in high yield by means of diethylaminoethyl cellulose chromatography of liver homogenate supernatant fluid, precipitation of the enzyme with protamine, selective extraction with ammonium sulfate solution, and rechromatography on the cellulose absorbent. Estimates of molecular weight made by several procedures, 76,900 f 5,000 (sedimenta...
متن کاملMechanism of action of guinea pig liver transglutaminase. I. Purification and properties of the enzyme: identification of a functional cysteine essential for activity.
Guinea pig liver transglutaminase has been purified 230fold in high yield by means of diethylaminoethyl cellulose chromatography of liver homogenate supernatant fluid, precipitation of the enzyme with protamine, selective extraction with ammonium sulfate solution, and rechromatography on the cellulose absorbent. Estimates of molecular weight made by several procedures, 76,900 f 5,000 (sedimenta...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 241 13 شماره
صفحات -
تاریخ انتشار 1966